Title Cell Encapsulation by Droplets
نویسندگان
چکیده
منابع مشابه
Title cell encapsulation by droplets.
Copyright © 2007 Journal of Visualized Experiments Page 1 of 1 Materials List for: Title Cell Encapsulation by Droplets Sangjun Moon, Pei-Ann Lin, Hasan Onur Keles, Seung-Schick Yoo, Utkan Demirci Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women's, Harvard Medical School Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineeri...
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INTRODUCTION Single-cell isolation is important for biochemical screening platform in which each cell can be genetically varied and observation of individual reaction level is necessary. Microfluidic droplet-based technology enables the isolation of cells in individual aqueous chamber, but single-cell encapsulation efficiency is usually as low as 22% [1]. Several reports have been presented to ...
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Single cell study is gaining importance because of the cell-to-cell variation that exists within cell population, even after significant initial sorting. Analysis of such variation at the gene expression level could impact single cell functional genomics, cancer, stem-cell research, and drug screening. The on-chip monitoring of individual cells in an isolated environment would prevent cross-con...
متن کاملTowards a High-throughput Electrofusion Platform Using Droplets: Cell Detection and Deterministic Encapsulation
Here, an innovative lab-on-a-chip platform towards electrofusion in droplets is described. It comprises a novel, impedance-based cell in droplet detection. This platform also enables label-free discrimination between viable and dead cells. Moreover, a new approach of efficient encapsulation of beads in droplets is presented. Bead encapsulation with efficiencies up to 95% is shown, using inertia...
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Oils containing both fluorescent semiconductor and magnetic oxide nanoparticles are used to produce oil in water emulsions. This technique produces oil droplets with homogeneous fluorescence and high magnetic nanoparticle concentrations. The optical properties of the oil droplets are studied as a function of the droplet sizes for various concentrations of fluorescent and magnetic nanoparticles....
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2007
ISSN: 1940-087X
DOI: 10.3791/316